Rapid evolution of mutation rate and spectrum in response to environmental and population-genetic challenges – Nature Communications
Long-term evolution experiments All populations originated from two ancestral strains with different initial mutation-rates. Ancestral wild-type (WT) are descendants of PFM2, a prototrophic derivative of E. coli K-12 str. MG1655, while ancestral mismatch repair knockouts (MMR-) are descendants of PFM5 (PFM2, ∆mutL)24. The long-term cultures consist of three transfer-size treatments, differing by 1 mL (large transfer-size …
